![]() ![]() ![]() Viral targets can be detected either separately with separate fluorophores or jointly using the same fluorophore, thus increasing the test’s reliability and sensitivity. We have used the MIQE guidelines to develop two versions of a unique fiveplex RT-qPCR test, termed CoV2-ID, that allows the detection of three viral target genes, a human internal control for confirming the presence of human cells in a sample and a control artificial RNA for quality assessment and potential quantification. ![]() work is licensed under a CC BY 4.0 LicenseStatus:PostedVersion 1posted 11 Sep, 2020You are reading this latest preprint versionAbstractAccurate, reliable and rapid detection of SARS-CoV-2 is essential not only for correct diagnosis of individual disease but also for the development of a rational strategy aimed at lifting confinement restrictions and preparing for possible recurrent waves of viral infections. CoV2-ID, a MIQE-compliant sub-20-minute 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19 | Research Square BrowsePreprintsCOVID-19 PreprintsProtocolsVideosJournalsTools & ServicesOverviewDigital EditingProfessional EditingBadgesResearch PromotionYour CartAboutPreprint PlatformIn ReviewEditorial PoliciesFAQOur TeamAdvisory BoardBlogSign InSubmit a PreprintCiteShareDownload PDFArticleCoV2-ID, a MIQE-compliant sub-20-minute 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19Stephen Bustin, Amy Coward, Garry Sadler, Louise Teare, Tania NolanThis is a preprint it has not been peer reviewed by a journal. ![]()
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